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Fig. 2. In situ hybridization of 35S labeled riboprobes to limb elements following Smoothened removal. (A-J) E14.5 tibial elements of indicated genotypes hybridized with probes as indicated. Signals are shown in red on sections counterstained with Toluidine Blue. The orange arrows denote the perichondrium not expressing the various genes in Cre10; Smon/c embryos.