Fig. 1. Fluorescence immunohistochemistry for Lim1 and Prox1 in stage 24-35 developing avian retina. Micrographs showing immunohistochemistry for (AH) Lim1 (green) in horizontal cells (HCs) and Ng-CAM (red) on ganglion cells in stage 24-35 retina. (I) Overview of Lim1 immunoreactivity in stage 35 retina. The gradual spatial maturation with corresponding developmental stages are indicated. (J-M) Prox1 (red) and Lim1 (green) immunoreactivity in (J, K) stage 28, (L) stage 33 and (M) stage 35 retinas. Arrows in I and J indicate the central region of the retina. st, stage. Scale bars: in A (for A-C), D (for D-H), K, L (for L-M) are 20 µm and I (for I-J) is 100 µm.