Fig. 5. Reduction of pMad is correlated with upregulated bam transcription in gbb mutant GSCs. All the germaria are labeled for pMad (red), GFP (green), Hts (blue, fusomes) and DAPI (white). The panels A'-F' represent the corresponding DAPI images for panels A-F. Circles highlight cap cells, whereas asterisks indicate GSCs. (A) A germarial tip from a bam-GFP female cultured at 29°C for 4 days showing normal pMad expression and no bam-GFP expression in GSCs. (B-F) Germarial tips from either bam-GFP gbb⁴/gbb^D4 (C,F) or bam-GFP gbb⁴/gbb^D20 (B,D,E) females cultured at 29°C for 4 days, showing reduced pMad expression. gbb mutant GSCs with easily detected pMad do not express bam-GFP (B,C; one indicated by an arrow in D), whereas the other GSCs with severely reduced pMad show bam-GFP expression (one indicated by an arrowhead in D). All the micrographs are shown at the same scale. Scale bar: 10 µm.