Fig. 7. Two Bmp like molecules, Dpp and Gbb, are expressed in the somatic cells in the Drosophila testis. The testes in A-C are labeled for Hts (red), GFP (green) and DAPI (blue), and their hub cells are highlighted by circles. (A) The tip of a upd-gal4;UAS-GFP testis showing GFP-labeled hub cells. (B) The tip of a c587-gal4;UAS-GFP testis showing GFP-labeled somatic stem cells and somatic cyst cells. (C) The tip of a vasa-GFP testis showing GFP-labeled germ cells including GSCs. (D) A DNA gel with RT-PCR products showing that gbb and dpp mRNAs are primarily present in the somatic cells of the testis. In this gel, mRNAs from the whole testes, purified germ cells, somatic cyst cells/somatic stem cells and hub cells are marked as templates 1, 2, 3 and 4, respectively. vasa serves as a positive control, while rp49 is an internal control. For dpp (10x), approximately 10-fold more RNA template was used because of its low abundance. A-C are shown at the same magnification. Scale bar: 10 µm.