Fig. 4. ph^lac mutant germaria exhibit abnormal encapsulation by prefollicular cells and abnormal budding of germline cysts. (A-G) DAPI staining (A-G,E'',F'') and anti-Fas III staining (D',E',E'',F',F'',G') of ph^lac mutant ovarioles. (A,B) arrows point to compound follicles, with the same degree (A), or different degree (B) of polyploidy of the nurse cells. (C) ph^lac follicle with fewer than 16 nurse cells (one nurse cell, arrow). No complementary chamber could be found, probably due to nurse cell degeneration (empty arrowhead points to pycnotic nucleus). (D-G) In ph^lac germaria, cysts are haphazardly distributed (D', arrowheads), and prefollicular cells fail to migrate, remaining at the periphery (D',G', asterisks). Encapsulation defects include splitting of germline cysts (E',E'', arrowhead and empty arrowhead point to follicles with fewer than 15 nurse cells, having the same degree of polyploidy as the last egg chamber in germarial region 3) or to several cysts encapsulated together (F',F''; arrows point to an absence of prefollicular cell migration between adjacent germline cysts, whereas arrowheads point to an invagination of prefollicular cells). (G) In strongly affected ovarioles, prefollicular cells completely failed to migrate (G', asterisks), leading to the inclusion of several germline cysts in the same follicle. Arrowhead points to a `highly multicystic' follicle.