Fig. 6. Mutations in the Sce¹ gene lead to ovarian defects. (A-E) Sce¹ mosaic follicles stained with DAPI (A,B), anti-Fas III (A'',B',C,C',E,E'), or anti--Spectrin (D,D') antibodies. Mutant clones for Sce¹ are detected by the absence of GFP (A',B',C,D,E, green). (A-A'') Compound follicle (A, arrows point to oocyte nuclei) bearing several Sce¹ clones marked by the absence of GFP staining (A') and exhibiting two pairs of polar cells at the anterior and posterior poles (A''). (B-) Mosaic Sce¹/Sce⁺ germarium. Sce¹ mutant prefollicular cells do not migrate correctly arround germline cysts (B', bracket). Arrowhead points to an abnormal interfollicular stalk with Sce¹ cells on another focal plane. (C,D) Interfollicular stalks are very long and all cells express Fas III (C,C') as well as -Spectrin (D,D') at high levels compared with adjacent follicle cells. Empty arrowhead (C) points to one Sce¹ cell (four other Sce¹ cells are present in this stalk). (E-E') Mosaic Sce¹/Sce⁺ follicle. Sce¹ follicular cells strongly express Fas III protein compared with adjacent Sce⁺ cells.