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Fig. 6. Overexpression of the RA isoform of PKA-R1 causes a reduction in PKA activity and Oskar protein accumulation. (A) Wild-type adult wing. (B) An ectopic wing outgrowth, which is observed when the RA isoform is overexpressed ubiquitously using an actinGAL4 driver. The penetrance of this phenotype is about 30%. (C,D) oskar RNA localization in stage 10 wild-type egg-chambers and in egg chambers in which RA was overexpressed using the actinGAL4 driver, revealed by in situ hybridization. oskar RNA localizes correctly at the posterior pole in wild type (C) and in RA-overexpressing oocytes (D). (E) Northern blot analysis of wild-type and 18304 hemizygote ovarian poly(A)+ RNA probed with an oskar cDNA probe (top panel). The blot was re-probed with rp49 cDNA as a loading control (bottom panel), revealing that there was no difference in oskar RNA expression levels between wild-type and RA-overexpressing ovaries. (F) Western blot analysis of protein extracts of wild-type and RA-overexpressing ovaries (actinGAL4 and nanosGAL4VP16), probed with anti-Oskar antiserum. Both isoforms of Oskar protein are expressed at significantly lower levels in the extracts of ovaries in which RA is overexpressed (top). The blot was re-probed with anti-{alpha}-Tubulin antibody as a loading control (bottom).