Fig. 2. Overexpression of hfp inhibits cell cycle entry in the developing eye and wing. (A,B) Scanning electron micrographs of adult eyes of wild type (A) and GMR-GAL4, UAS-hfp/+; UAS-hfp/+ (B). Scale bar equals 200 µm. (C-F) Eye imaginal discs from wild type (C,E) and GMR-GAL4,UAS-hfp/+; UAS-hfp/+ (D,F) third instar larvae, co-labelled with BrdU (C,D) and anti-phosphohistone H3 antibody (E,F). Posterior is to the left. Yellow bars indicate the MF. (G-J) Adult wings mounted in Canada balsam (G-I) or fresh (J) from en-GAL4,UAS-GFP (G) and en-GAL4,UAS-GFP/+,UAS-hfp/+ (H-J) flies. (K-N) Third instar wing discs from en-GAL4,UAS-GFP (K,M) and en-GAL4,UAS-GFP/+,UAS-hfp/+ (L,N) flies, co-labelled using GFP antibody staining to mark the posterior region of the wing disc (K,L) and BrdU (M,N). The ZNC is marked with an arrow, and in (N) the GFP-positive region is outlined in white.