Fig. 4. hfp^EP dominantly suppresses the dmyc mutant ovary phenotype and dmyc expression is increased in hfp mutant clones. (A-T) All ovarioles are oriented with the most mature/posterior egg chamber to the right. nc=nurse cells, af=actin filament bundles, fc=follicle cells. (A-B) Ovaries stained with phalloidin (red) to show filamentous actin and (C-H) with phalloidin and the DNA stain Oligreen. Egg chamber genotypes and stages: wild-type stage 10 (A), dmyc^P0/dmyc^P0 stage 10 (B), wild-type stage 11 (C), dmyc^P0/dmyc^P0 stage 11 (D), dmyc^P0/dmyc^P0; hfp^EP/+ stage 11 (E), wild-type stage 14 (F), dmyc^P0/dmyc^P0 arrested at stage 11 (G), dmyc^P0/dmyc^P0; hfp^EP/+ stage 14 (H). (I-N) Ovarioles containing stage 10B egg chambers, labelled with BrdU (green) to visualize chorion gene amplification and counterstained with the DNA stain propidium iodide (red in L-N). Genotypes: wild type (I,L), dmyc^P1/dmyc^P1 (J,M), dmyc^P1/dmyc^P1; hfp^EP/+ (K,N). (O-T) Ovarioles containing stage 10 egg chambers, showing in-situ hybridization to dmyc mRNA (red) and counterstained with Oligreen. Genotypes: wild type (O,R), dmyc^P0/dmyc^P0 (P,S), dmyc^P0/dmyc^P0; hfp^EP/+ (Q,T). (U-Z) Analysis of dmyc mRNA in third instar wing discs, the ZNC is marked with a yellow bar. (U) wild-type dmyc in-situ pattern, (V-Z) hs-FLP/+; FRT80Bhfp^EP/FRT80B Tb-GFP, (V) hfp^EP/hfp^EP clones marked by the absence of GFP antibody staining and outlined in white, (W) dmyc mRNA expression in hfp mutant clones, (X) merged image. (Y,Z) high power images of hfp^EP/hfp^EP mutant clones; (Y) GFP antibody staining and (Z) dmyc in situ.