Fig. 6. Hfp negatively regulates Stg. (A-H) Cycle 16 embryos stained with anti-phosphohistone H3 in green to detect mitotic cells and anti-Actin in red to show cell cortex. (A,B) wild type, (C,D) hfp^EP mutant embryos (E,F) stg^AR2 mutant embryos and (G,H) hfp^EP, stg^AR2 double-mutant embryo. (I-N) Wing discs from third instar hs-FLP; FRT80Bhfp^EP/FRT80BTb-GFP flies. (I,L) hfp mutant clones are marked by the absence of GFP and outlined in white, (J) in-situ hybridization of stg mRNA, (K) in situ merged with GFP, (M) staining with anti-stg antibody and (N) stg-antibody staining merged with GFP.