Fig. 3. hunchback is maternally expressed and loaded into developing oocytes. (A) Northern blot using a hunchback probe to ovarian (O) or embryonic (E) total RNA. A single band of 3.2 kb is detected. (B) In situ with hunchback probe on a single fixed ovariole. Arrowhead indicates zone III of tropharium, the area where the nurse cells are concentrated. Older oocyte (arrow) beginning to lay down chorion does not stain as strongly. (C) Ovariole in situ using sense control hunchback probe.