Fig. 10. Zic3 misexpression did not alter the expression of netrin 1 and CSPGs. The RCAS-Zic3-infected samples were hybridized with the netrin 1 probe (A) and then immunostained with an anti-GAG antibody (p27) (B). Netrin 1 is highly expressed in the optic disc (OD, in A) and optic fissure, similar to wild-type uninjected retinas. The RCAS-Zic3 virus-infected retinas were also double stained with an anti-chondroitin sulfate antibody (CS-56) (C,E) and an anti-viral GAG antibody (P27) (D,F). CS-56 staining is intense at the periphery of the retina. However, a lower level of CS-56 staining is also visible in the center of the retina (E). Comparing the RCAS-Zic3 virus-infected area (arrowheads in D,F) with the adjacent uninfected areas, there is no difference in the level of CS-56 expression (C-F). Identical fields are shown in A and B, C and D, E and F, respectively. Scale bar: 200 µm.