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Fig. 2. Expression of Zic3 mRNA in whole-mount chicken embryos (A-C) and flat-mount retinas (D-G) by in situ hybridization. (A) HH stage 10; (B) HH stage 12; (C) HH stage 17. Zic3 is expressed in early optic vesicle (arrows in A and B) and optic cup (arrow in C). (D) E5.5 retina; (E) E7 retina; (F) E9 retina; (G) high-magnification view of E7 retina. Zic3 is expressed in a periphery-to-center gradient with higher expression at the periphery. All the flat-mount retinas were shown with the ganglion cell side upwards, oriented with the anterior side (A) towards the left, the posterior side (P) towards the right, the dorsal side (D) upwards and the ventral side (V) downwards.