Fig. 7. Misexpression of Zic3 did not grossly alter retinal lamination. Cryosections of E10 retinas infected with RCAS-Zic3 were stained with anti-neurofilament (A-C) and various cell type-specific antibodies including: anti-visinin (G), anti-islet 1 (H) and anti-Pax6 (I). (C-F) Same RCAS-Zic3-infected retinal section was shown stained with anti-neurofilament (C), DAPI (D), anti-viral GAG antibody (F) and in phase contrast (E). Note some axons in the RCAS-Zic3-infected retinas misrouted to the subretinal spaces at the photoreceptor side of the retina (arrowheads, A-C). However, the laminated structure of the retina was largely normal (D-I), except some dispersion of cells towards the photoreceptor side (arrowheads, H and I). The optic fiber layer was largely normal, except for some protrusions of the axons (arrows in A,B). Frequently, however, the photoreceptor layer was broken (arrowheads, D and G). Note that the anti-viral GAG antibody staining was much more intense at the sites of axon crossing-over than the nearby infected region (arrowhead in F). PR, photoreceptor layer; OFL, optic fiber layer; GCL, ganglion cell layer; INL, inner nuclear layer.