Fig. 1. Defective cumulus expansion in Ptx3^–/– mice. (A-D) Morphology of cumuli oophori during matrix deposition. Micrographs show individual preovulatory follicles 10 hours after hCG (A,B) and an enlargement of the enclosed cumuli (C,D). (E-H) Morphology of Ptx3^+/– and Ptx3^–/– ovulated cumuli immediately after isolation (E,F), or after 1 hour of in vitro culture (G,H). Scale Bars: 100 µm in A,B,E-H; 50 µm in C,D.