Fig. 3. PTX3 synthesis by in vitro cultured cumulus cells. (A) COCs (30/20 µl) were cultured for 16 hours in the absence (BM, basal medium) or presence of the indicated stimuli. (B) Cumulus cells isolated from 30 COCs were cultured for 16 hours in the presence of 60 oocytes or FSH, or both. PTX3 was assessed in the matrix and medium by western blotting.