Fig. 6. Co-localization of PTX3 and HA in ovulated cumuli. Ptx3^+/– cumuli were stained for HA (red), PTX3 (green) and chromatin (blue), as reported in Materials and methods, and the innermost (A-D) and outermost (E-H) layers of a cumulus were photographed using a fluorescence microscope. HA histochemical staining (A,E); PTX3 immunostaining (B,F); merging of the two signals (C,G); and staining of cumulus cell nuclei (D,H). Cumuli incubated with secondary probes only showed no specific staining (data not shown).