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Fig. 9. Expression of PTX3 in human ovarian tissues. (A) PTX3 mRNA expression by human cumulus cells in four different patients undergoing IVF. Each lane was loaded with 10 µg of total RNA. Ethidium bromide staining of the gel is shown in the lower panel. (B) Detection of PTX3 in human cumulus matrix by western blot analysis. Lane 1, human cumuli; lane 2, recombinant PTX3. (C) PTX3 protein levels in the follicular fluid of five different patients undergoing IVF. The dotted line indicates PTX3 plasma levels in healthy donors.