Fig. 3. Effects of hemolymph injection on the death of wing epidermal cells in en-Gal4 UAS-GFPN flies. Hemolymph collected from wild-type flies at various times after eclosion was injected into flies neck-ligated at eclosion and wings were examined for induction of the cell death 2 hours later (A-C). Cell death was not induced by injection of hemolymph from flies at eclosion (A), but was induced by it at 30 minutes after eclosion (B). Hemolymph from flies at 30 minutes after eclosion was injected into pharate adults neck-ligated at various stages and induction of the cell death was examined 2 hours after injection (D-F). Injection of hemolymph into the flies at S stage did not induce cell death (D), but it did at W stage (E). (C,F) Black and gray bars indicate the percentage of flies showing cell death extensively or in restricted domains of the wing, respectively. The number of flies examined is shown in parentheses.