Fig. 2. Spatial and temporal expression patterns of Barhl genes during mouse retinogenesis. (A,B) In situ hybridization analysis of E14.5 and P1 retinal sections using a Barhl2 antisense probe showed prominent signals in the inner retina. (C-F) Retinal sections from the indicated developmental stages were immunostained with an anti-Barhl2 antibody. In embryonic, postnatal and adult retinas, the anti-Barhl2 antibody labeled many cells in the inner nuclear and ganglion cell layers (C-F), as well as some migratory cells within the outer neuroblastic layer (C,D). Arrows indicate representative differentiating and mature horizontal cells. (G) In situ hybridization analysis of E16.5 retinal sections using a Barhl1 antisense probe detected no signals in the retina. (H) X-gal staining of retinal sections from E16.5 Barhl1^lacZ/+ embryos counterstained with Fast Red. No ß-galactosidase activity was detected in the retina. GCL, ganglion cell layer; inbl, inner neuroblastic layer; INL, inner nuclear layer; IPL, inner plexiform layer; IS, inner segment; onbl, outer neuroblastic layer; ONL, outer nuclear layer; OPL, outer plexiform layer; OS, outer segment. Scale bar: 25 µm.