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Fig. 7. Role of epithelial and mesenchymal ER{alpha} in the disruption of p63. Ep, epithelium. St, stroma. BV, blood vessel. (A) Four types of tissue recombinants were constructed with UtE and VgM from P2 ER{alpha}+/+ and ER{alpha}–/– mice (ER{alpha}+/+ UtE+ER{alpha}+/+ VgM, ER{alpha}+/+ UtE+ER{alpha}–/– VgM, ER{alpha}–/– UtE+ER{alpha}+/+ VgM and ER{alpha}–/– UtE+ER{alpha}–/– VgM) and grafted into ovariectomized female nude mice hosts without (a,b; control) or with (c-f; +DES) DES-treatment. Without the DES-treatment, p63-positive basal layer was induced in all 4 types of tissue recombinants (a,b). When ER{alpha} was expressed in the epithelial cells [ER{alpha}+/+ UtE+ER{alpha}+/+ VgM (c) and ER{alpha}+/+ UtE+ER{alpha}–/– VgM (e)], induction of p63 in the UtE was blocked by DES. By contrast, induction of p63 and formation of squamous basal cells were not affected by DES in ER{alpha}–/– UtE+ER{alpha}+/+ VgM (d) and ER{alpha}–/– UtE+ER{alpha}–/– VgM (f) tissue recombinants. The results were quantitated by measuring the length of basement membrane associating with p63-positive and -negative epithelial cells (B). The result was statistically analyzed with ANOVA test. Error bars represent s.e.m. The bar marked with b is significantly higher than bars marked with c and d (P<0.05). The bars marked with a, b and c are significantly higher than bars marked with d (P<0.01). Scale bar: 100 µm.