Fig. 5. Analysis of kidney capsule grafts of activin-stimulated GFP-Bry⁺ and GFP-Bry^– EB-derived populations. GFP-Bry⁺ and GFP-Bry^– cells isolated from day 5 EBs differentiated in the presence of 3 or 100 ng/ml activin were reaggregated and cultured as EBs for an additional 8 days in SF cultures in the absence of factor. The reaggregated EBs were replated in hepatocyte conditions for 4 days and then harvested and transplanted under the kidney capsule of SCID-beige mice. Three weeks following transplantation, the mice were sacrificed and the kidneys harvested for analysis. (A) Photograph showing the size of the grafts from the GFP-Bry⁺ and GFP-Bry^– populations. Size is indicted by the ruler (mm) at the bottom of the figure. (B) Histological analysis of grafts from the GFP-Bry⁺ and GFP-Bry^– populations. SM, skeletal muscle; Gu, gut epithelial-like structure, B, bone; C, columnar cells; Br, neural tissue; NT, neural tube-like structure.