Fig. 7. Differential requirement for topi and achi/vis in expression of some target genes. (A) RT-PCR of CG8349 from wild-type and meiotic arrest mutant testes. No-RT is a negative control, gDNA is PCR on genomic DNA from wild-type flies. Two exposures of the same gel are shown to emphasize the differences between the mutants and wild type (top), and the lack of product in achi/vis (bottom). (B-E) In situ hybridization of CG8349 probe to testes revealed strong expression in wild-type (B) primary spermatocytes, persisting until mid-late elongation stages, weaker expression in comr (C) and topi (E), and no detectable signal in achi/vis (D) testes. (F) RT-PCR of TrxT from wild-type and meiotic arrest mutant testes. No-RT is a negative control, gDNA is PCR on genomic DNA from wild-type flies. (G-J) In situ hybridization of TrxT probe testes revealed strong expression in wild-type (G) primary spermatocytes and early-mid elongation stages. The signal in achi/vis testes was of a similar intensity to wild type (I). Weaker expression was seen in comr (H) and there was no detectable signal in topi (J) testes.