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Fig. 2. Regulation of the Foxh1-dependent (n2)7-luc reporter by Smad2 or Smad3. (A) The constitutively active Alk4 (caAlk4) Nodal receptor with or without mycSmad4 leads to modest upregulation of the (n2)7-luc reporter in Mv1Lu cells. Addition of either FLAG-hSmad3 or FLAG-hSmad2 potentiates reporter activity to levels consistent with the previously demonstrated TGFß-dependent regulation of (n2)7-luc (Saijoh et al., 2000). (B) Western blot analysis of protein extracts from transfected cells confirms appropriate protein production of mycSmad4, FLAG-hSmad3 and FLAG-hSmad2 proteins.