Fig. 7. Charon is required for the early processes of L/R patterning. (A-I) Embryos were injected with charon-MO and examined for atv/lefty1 (20 hpf, A-C), spaw (18 hpf, E-F), and pitx2 (20 hpf, G-I) expression. In addition to the normal (left-sided) expression of the gene (A,D,G), bilateral (B,E,H) or reversed (C,F,I) expression of the left-side-specific markers was also observed. The numbers of embryos showing each phenotype are given in Table 3. Embryos that showed strong right-side stain and weaker left-side stain were recorded as bilateral (dorsal views). Arrowheads indicate the presence of the expression. (J-T) Midline barriers are not affected in charon morphant embryos. no tail (ntl) expression in notochord of wild-type (J,K) and charon morphant embryos (L,M) at 13 hpf. sonic hedgehog (shh) expression in notochord at 13 hpf (N,O) and in floor plate at 24 hpf (R,S) in wild-type (N,R) and charon morphant embryos (O,S). antivin/lefty1 expression in wild-type (P) and charon morphant embryos (Q) at the 6-9-somite stage. Saggital sections of ntl expression at 24 hpf in wild-type (T) and charon morphant embryos (U). (J,L,R,S) Lateral views. (K,M,N,O) Dorsal views. (P,Q) Dorso-lateral views.