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Fig. 4. Influence of cholesterol, food, and pheromone on daf-9 expression of L3. (A) N2 dhIs64 grown on normal nematode growth (NG) media. (B) N2 dhIs64 grown on NG lacking added cholesterol (NG – chol). (C) N2 dhIs64 dauer larva grown on NG agarose lacking cholesterol (– chol). (D) daf-12(rh61rh411) dhIs64 grown on NG lacking cholesterol. (E) Hypodermal daf-9 expression with increasing dilutions of 2% DH5{alpha}. (F) Hypodermal daf-9 expression at different concentrations of pheromone. Hypodermis (arrowheads) and XXXL/R cells (X). daf-9::gfp intensity (–, +, ++, +++), see Fig. 3. Worms are cultured at 20°C.