Fig. 5. Analysis of proliferation in back skin of (A) wild-type or (C,E) D2 transgenic mice and in (B,D,F) paw skin of D4 transgenic mice. (A-D) BrdU labelling (green) with propidium iodide nuclear staining (red). Green fluorescence of hair shafts is non specific. (E,F) Cyclin D1 immunohistochemistry (brown) with Haematoxylin counterstain (blue). Mice were treated with 4OHT for the periods indicated. Arrows in C and E indicate outgrowths arising from the hair follicles that are positive for BrdU and Cyclin D1, respectively. Scale bars: 100 µm.