Fig. 6. Expression of hair follicle differentiation markers. (A-D) Untreated wild-type anagen follicles. (E-H) Dorsal back skin of D2 transgenic mice treated with 4OHT for the periods indicated. (I-L) Dorsal paw skin of D4 transgenic mice treated with 4OHT for 5 days. Paraffin sections were immunostained with (A,E,I) anti-keratin 17, (B,F,J) anti-CDP, (C,G,K) anti-trichohyalin, or (D,H,L) anti-Lef1 antibodies and counterstained with Haematoxylin. Arrows in F and H indicate positive staining in the epithelial outgrowths from pre-existing follicles. Scale bar: 100 µm.