Fig. 8. Effects of transient ß-catenin activation in K14Nß-cateninER transgenic mice. (A) Schematic representation of experiments performed with the D2 and D4 transgenic lines. The arrow represents time in days (not to scale). Dots are time points analysed. Continuous lines connecting dots represent periods of daily 4OHT treatment and dashed lines indicate no 4OHT treatment. (B-G) Mice received the number of doses (d) of 4OHT indicated and were kept without 4OHT for number of days shown (+). (B,C,E-G) Back skin, (D) paw skin. Arrows in B and C indicate epithelial outgrowths from the permanent portion of the hair follicles and from sebaceous glands. Arrows in D indicate new hair buds arising from interfollicular epidermis. (H-Q) expression of cyclin D1 (H), Lef1 (I,L), keratin 17 (J,M), keratin 1 (K) and ß-catenin (green, N-Q; red fluorescence is propidium iodide counterstain) in D2 tumours, after 28 days of 4OHT treatment (H-J,N,O) or 28 days of treatment followed by 28 (P,Q) or 106 days (K-M) without 4OHT. Arrows in I highlight Lef1 expression. O,P are high magnification views of boxed regions in N, Q respectively. Scale bars: 100 µm (A-M), 50 µm (N-Q).