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Fig. 4. Tbx1 determines the position of neural bHLH gene expression domain borders. (A) Expression domain morphologies (colored regions) revealed by 3-D reconstruction of serial section sets. Otocysts are shown in ventral view and rendered transparently to visualize dorsal Tbx1 expression. Models are normalized for AP length across stages. (B) 3-D reconstructions of the otocyst showing the Ngn1 expression domain (green) at E10 for each genotype.