Fig. 2. Increased proliferation of epithelial cells in explants treated with Wnt CM. (A,B) BrdU incorporation assays demonstrated stimulation of the cell cycle in explants treated with Wnt 3a CM. (C,D) Nuclei of explants derived from rat lens capsule revealed by Hoechst 33258 staining. (E) Quantification of BrdU incorporation after a 6-hour labeling period. BrdU-positive nuclei from a total of six explants treated with each factor were counted. The mean values (±s.d.) are shown in the histogram. (n=5) [versus control (Cont), *P<0.005].