Fig. 8. Wnt CM induces the accumulation of lens fiber proteins in FGF2-stimulated epithelial cells. Explants pre-stimulated by FGF2 for 1 hour were washed and cultured in control medium or medium supplemented with Wnt3a CM and incubated for 5 days. Explants were analyzed by immunolabeling with antibodies against ß-crystallin (A), aquaporin 0 (B) or were stained with Hoechst 33258. Explants cultured in control medium did not accumulate ß-crystallin or aquaporin 0, but these fiber-specific markers accumulated in cells exposed to Wnt CM. Scale bar: 50 µm.