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Fig. 3. Iron deficiency anemia at birth and iron accumulation in young adults in Pcm mutant mice. (A) Peripheral blood smear remarkable for hypochromic, microcytic red blood cells in Pcm/Pcm mice at P0. Wright-Giemsa stain. (B) Prussian Blue staining of P0 liver sections reveals decreased parenchymal liver iron in Pcm/Pcm mice. (C) Quantification of total iron at P0 demonstrates organismal iron deficiency in Pcm mutant pups. (D) Prussian Blue staining of sections of 12-week-old liver shows numerous localized iron deposits in Pcm/Pcm mice. (E) Hepatic iron content significantly elevated by 12 weeks of age in Pcm/Pcm mice. (F) Decreased serum iron levels in Pcm mutants compared with wild type. Asterisk, P<0.05; double asterisk, P<0.01; triple asterisk, P<0.001.