Fig. 5. Altered Fpn1 protein and Hamp mRNA expression in Pcm mutant mice. (A) Western blot analysis reveals graded increase in Fpn1 protein expression in P0 mutant liver, with concomitant graded decrease in ferritin expression. Approximate molecular masses: Fpn1, 68 kDa; ferritin (Ftn), 20 kDa; actin, 41 kDa. (B) Three-week-old liver shows similar graded increase in Fpn1 protein expression on a western blot. (C) Seven-week-old liver exhibits persistence of Fpn1 protein expression only in polycythemic Pcm heterozygous mice on a western blot. (D) Seven-week-old duodenum demonstrates pattern of Fpn1 protein expression similar to liver on a western blot. (E) Twelve-week-old liver reveals downregulation of Fpn1 protein expression in all genotypes on a western blot. (F) Seven-week-old liver RT-PCR shows no significant difference in Fpn1 mRNA expression, but lack of Hamp mRNA expression in polycythemic mutant mice. Band sizes: Fpn1, 338 bp; Hamp, 171 bp; ß-actin, 250 bp. (G) Twelve-week-old liver RT-PCR demonstrates upregulation of Hamp mRNA expression in all animals.