Fig. 2. Development of megakaryocytes and myeloid cells. (A,B) May-Giemsa staining (A, left cell) and CD41 (Cy3) staining (B) of megakaryocytes on day 12. Nuclei were labeled with Hoechst 33342 in B. (C,D) May-Giemsa staining (C) and myeloperoxidase staining (D) of myeloid cells on day 18. Scale bars: 10 µm. (E) Sequential analysis of the percentages of erythrocytes (white columns), myeloid cells (striped columns) and megakaryocytes (black columns) among total hematopoietic cells in the presence of EPO. Each column represents the mean of triplicate wells. Representative results from one of three independent experiments are shown.