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Fig. 6. Sequential flow cytometric analysis of KIT, FLK1 and CD34 during differentiation induction. Undifferentiated ES cells, or the cultures on day 4, 6, 12 and 18, were stained with antibodies specific for KIT, FLK1 or CD34, or with control IgG1. Plots show the isotype control IgG1 staining profile (dashed lines) versus the specific antibody staining profiles (solid lines). Representative results from one of three independent experiments are shown.