Fig. 2. Characterization of ovarioles from females expressing the TkvAct receptor. All females were of genotype P{UAS.p-TkvAct}/P{Gal4::VP16-nos.UTR}. (A) Nomarski image of multiple tumorous ovarioles. (B) Confocal projection of tumorous ovariole from 2-day-old female carrying the Dad-lacZ transgene, indicating all germ cells express Tkv and Dad-lacZ (anti-Tkv, green; anti-lacZ, red). (C) Tumorous ovariole from a 1-day-old female indicating lack of BamC expression (anti-BamC, green; anti-spectrin, red). (D,E) Tumorous ovarioles from 3-day-old (D) and 1-week-old (E) females (anti-Hts, green; anti-Vasa, red). Arrow indicates position of terminal filament. Scale bars: 10 µm.