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Fig. 9. HPLC profiles showing CS-derived disaccharides (A). The largest peak, migrating at ~4 minutes retention time, represents unsulfated {triangleup}Di-0S, followed by the {triangleup}Di-4S disaccharide. Each tracing represents material from ~40 larvae. (B) CS-derived disaccharides from sotv and ttv mutants, shown as a percentage of wild-type controls (fraction of ng disaccharide/mg dry tissue, Ore R control). Unsulfated ({triangleup}Di-0S) and monosulfated disaccharides ({triangleup}Di-4S) across the sotv allelic series show changes in total CS levels as well as the degree of sulfation.