Fig. 5. Otu interacts with Hrb27C and plays a role in regulating grk localization. (A) Western blot probed for Hrb27C after immunoprecipitation (IP) of ovarian lysate using either Otu or Odd skipped (Odd) antibody. Only the Otu-IP shows a specific interaction. WCL, whole cell lysate (one-tenth volume). (B) A representative dorsalized egg laid by an otu⁷ mutant. (C) grk in situ hybridization of a late stage 9 egg chamber from an otu⁷ mutant. In wild type, grk is tightly localized to the dorsal anterior region of the oocyte (see Fig. 2E), but is mislocalized along the anterior circumference of the egg chamber in otu⁷ and otu¹¹ mutants.