Fig. 3. (A-E) Characteristic examples of RNA blot analyses. Each blot consists of 3 µg polyA⁺ RNA per lane from female (F) or male (M) 5-day-old adult animals: wild type (WT) and tud. Microarray values for each gene (see Materials and methods) are included below the respective lanes. Probes were derived from ESTs for female genes (A-C) or male genes (D-E). (A) CG17012, (D) CG2858 and (E) CG8708 agree with microarray data. (B) CG7777 agrees with microarray data in that there is somatic expression (slight elevation in expression of female over male tud lanes). Germline expression is also apparent (much greater intensity of wild-type female over tud female bands). (C) CG7899 expression is sex-specific, but is due to germline rather than somatic expression. (F) Internal controls. Two genes, each with similar levels of female and male expression according to the microarray data, were used as internal control probes: CG4659 (Srp54K) (upper band) and CG10664 (cytochrome c oxidase subunit IV). Expression levels in the wild-type female lane of CG4659 and the tud male lane of CG10664 are typically slightly elevated.