Fig. 5. Temporal and tissue-specific expression in male internal genitalia (A) Schematic of the internal male reproductive system. Modified, with permission, from Miller (Miller, 1950). (B) Expression profiles of male genes (see Fig. 2 legend) (C) In situ hybridization of wild-type 5-day-old (I-VI), or 0-24 hour (VII), adult and mutant tud male reproductive tissues using probes derived from male genes. I-V part a, VII part a, and II parts e and f are frozen tissue frontal sections of whole animals; all other images are of whole-mount tissues. (I) Expression in the accessory glands: CG18284 (a-c), CG17022 (d) and CG17843 (e). (II) Expression of CG8708 in the anterior ejaculatory duct (a-d; c and d are different focal planes), including the papilla (d), and, sex-nonspecifically, in adult and embryonic salivary glands (e,f). (III) Expression of CG2858 in the ejaculatory bulb (a-e), showing individual differences in expression (c-e). (IV) Expression in accessory glands and the ejaculatory bulb: CG12558 (a-d) and CG9519 (e). (V) mfas expression in testes (a-c) and on the surface of seminal vesicles where the testes attach (c); in accessory glands (a,b,d); and in the ejaculatory bulb (b,e). (VI) prd expression in testes (T) and accessory glands (A). (VII) Expression of CG6788 in 0-24 hour ejaculatory bulb (a-c). Insets are digital magnifications.