Fig. 3. BC motility assay. (A1-A3) Wild-type stage 9 egg chambers of increasing maturity. As the border cells (BCs, arrow) move to the oocyte, oocyte area increases relative to the rest of the egg chamber (B,C,E). This oocyte growth can thus be used as a clock to measure the rate of BC migration. The % oocyte area (=oocyte area divided by the total area of the egg chamber; 23%) was plotted against the % migration (migration distance divided by the path distance; i.e. 66%). The results of from 400 wild-type egg chambers are shown in E. For all genotypes: 0.75r0.88, P<0.001. (F) Graphs for wild-type, rosy and Fas2^null BC clusters are shown. Delamination time is the point at which the BCs completely leave the epithelium (0% migration; green arrows). Migration time is the difference between the completion time (100% migration; red arrow) and the delamination time (green arrows). (D) BA3-Gal4 expression pattern. Slbo-Gal4 expression pattern is shown in Fig. 2D.