Fig. 3. Yeast origin ARS1 and its B2 element cannot functionally replace ori-ß. (A) Yeast origin ARS1 and its DNA unwinding element B2 were cloned into the BP construct replacing the entire ori-ß to generate constructs BP-ARS1 and BP-B2, respectively. (B) Quantitation of amplification levels for independent transgenic lines. BP, Big Parent line 3; OD, the ori-ß total deletion line 1.