Fig. 9. Disruption of RAM organization in the hlr mutant is not caused by the defective degradation of cyclin B1 or PIN4. Marker gene expression and localization of putative target proteins for proteasome-mediated degradation in 36-hour-old (A,B,E,F,I,J) and 10-day-old (C,D,G,H,K,L) seedlings of wild type (A,C,E,G,I,K) and hlr-1 mutants (B,D,F,H,J,L). (A-D) GUS staining of cycB1::GUS, a marker for the degradation of cyclin B1. (E-H) Whole-mount in situ immunolocalization of the PIN4 protein, a putative auxin efflux carrier. (I-L) Expression of DR5::GFP, a marker for auxin accumulation. Scale bars: 25 µm.