Fig. 9. Expression of hedgehog family members in Shh^-/-; Fkbp8^-/- mutants. (A) Neither Indian hedgehog (Ihh) nor desert hedgehog (Dhh) expression is detected in the vicinity of the E11.5 Shh^-/-; Fkbp8^-/- double mutant neural tube by in situ hybridization, although their endogenous expression (in the gut and gonad, respectively) is readily detected. (B) Hedgehog proteins are monitored using the monoclonal antibody 5E1. No staining is found within or around the E10.5 double mutant neural tube, although SHH protein is clearly detected in the notochord and floor plate of the wild-type embryo. Scale bars: 65 µm.