Fig. 7. Gene specific RT-PCR analyses of VEGFA receptors in epiphyseal chondrocytes. Total RNA was extracted from epiphyseal chondrocytes. RT-PCR reactions were performed in the presence of reverse transcriptase (RT) (+) or in its absence (–) (as a control for genomic DNA contamination). Cd31 was also amplified as a control for endothelial cell contamination. Nucleotide size markers are indicated at left. Amplified bands are only seen in the Vegfr3, Nrp1 and Nrp2 lanes, as indicated by asterisks.