Fig. 2. Genomic map of parkin locus, P-element mutagenesis and eclosion analysis of parkin mutants. (A) Locations of predicted genes and P-elements surrounding the parkin locus are shown. Solid boxes represent Drosophila genes and putative ORFs. The parkin rescue (dpkR) and stop-rescue (dpkSR) transgenes are identical except that dpkSR contains stop codons in all three reading frames of parkin. xxx indicates the position of the stop codons. (B) Generation of a null parkin allele. The dpk^P30 element was mobilized to generate the dpk^P21 insertion. The resulting double insertion chromosome was then used to delete the entire parkin-coding region while the surrounding genomic DNA remained intact. (C) Eclosion analysis. Progeny of w; dpk²¹/TM3 self cross was analyzed. Although little or no pre-pupal lethality was observed, 68% of parkin mutant pupae fail to eclose (92% of the dead pupae are parkin mutants).