Fig. 7. parkin mutants do not show dopaminergic neuron loss on whole-mount analysis of dopaminergic dorsomedial cluster (DMC) neurons. (A) Example of a morphologically normal Drosophila brain stained with anti-tyrosine hydroxylase antibodies with a higher magnification of DMC cluster neurons shown in B. (C) Dopaminergic cell counts of parkin mutant animals (w; dpk²¹) at 2 and 21 days after eclosion do not show any reduction in numbers of tyrosine hydroxylase-positive cells in dorsomedial clusters compared with heterozygous controls (w; dpk²¹/+).