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Fig. 5. Hand1 expression in yolk sacs is not regulated by Hand2. The expression of Hand1 was examined in yolk sacs of Hand1ß-gal/+Hand2-/- mice by staining for ß-gal activity. Wild-type (A) and Hand2-null embryo (B) showed similar expression patterns of Hand1. Hand1 was broadly expressed in the mesoderm of Hand2 null yolk sacs (C). All embryos are to the same scale. ht, heart; lm, lateral mesoderm; ys, yolk sac.