Fig. 7. Brg1 physically interacts with xNgnr1 and xNeuroD. (A) HeLa cells were transfected as indicated and applied to co-IP assay. Lysates were immunoprecipitated (IP) with anti-Myc antibodies and immunoblotted (IB) with anti-FLAG antibodies. Protein expression levels were monitored by western blotting of direct lysates. (B) Myc-xBrg1, FL-xBra, FL-xESR1, FL-xNgnr1 and FL-xNeuroD proteins were produced separately using reticulocyte lysate. After in vitro translation, an equal amount of lysate containing Myc-xBrg1 was added to FLAG-tagged proteins and subjected to co-IP with anti-Myc antibodies. Input of FLAG-tagged proteins was monitored by western blotting. Asterisks in B indicate specific bands corresponding to Ngnr1 and NeuroD.